Douche-Aourik, Fatima, Berlier, Willy, Féasson, Léonard et al. · Journal of medical virology · 2003 · DOI
Researchers tested muscle tissue samples from patients with fibromyalgia/ME/CFS and inflammatory muscle diseases to see if they contained enterovirus (a type of virus). They found viral genetic material in some patient samples but not in healthy people, though they did not find evidence of active viral proteins. This suggests that if a virus is present, it may be dormant or not fully active in muscle tissue.
This work contributes to understanding whether persistent viral infections might underlie ME/CFS muscle pathology. Detection of viral RNA without active viral proteins could explain chronic symptoms without obvious ongoing inflammation, and supports the hypothesis that muscle tissue may harbor dormant enteroviral infections in ME/CFS patients.
This study does not prove that enterovirus causes ME/CFS or fibromyalgia, only that viral RNA is more common in affected patients than controls. The absence of VP-1 protein means the virus is not actively replicating, so the clinical relevance of finding dormant viral RNA remains unclear. A small sample size and cross-sectional design prevent definitive conclusions about causation or disease mechanisms.
About the PEM badge: “PEM required” means post-exertional malaise was an explicit required diagnostic criterion for participant inclusion in this study — not that PEM was studied, observed, or discussed. Studies using criteria that do not require PEM (e.g. Fukuda, Oxford) are tagged “PEM not required”. How the atlas works →
The first block is for the primary paper and is the citation you should use in research work. The atlas-snapshot line only applies if you are specifically referring to this atlas’s reading of the paper on the date shown.
Primary citation
Douche-Aourik, Fatima, Berlier, Willy, Féasson, Léonard, Bourlet, Thomas, Harrath, Rafik, Omar, Shabir, et al. (2003). Detection of enterovirus in human skeletal muscle from patients with chronic inflammatory muscle disease or fibromyalgia and healthy subjects.. Journal of medical virology. https://doi.org/10.1002/jmv.10531
BibTeX
@article{mecfsatlas-douche-aourik-2003-detection-enterovirus,
author = {Douche-Aourik, Fatima and Berlier, Willy and Féasson, Léonard and Bourlet, Thomas and Harrath, Rafik and Omar, Shabir and Grattard, Florence and Denis, Christian and Pozzetto, Bruno},
title = {Detection of enterovirus in human skeletal muscle from patients with chronic inflammatory muscle disease or fibromyalgia and healthy subjects.},
journal = {Journal of medical virology},
year = {2003},
doi = {10.1002/jmv.10531},
note = {PubMed: 14556267},
url = {https://www.mecfsatlas.com/evidence/douche-aourik-2003-detection-enterovirus},
}Atlas snapshot reference
ME/CFS Atlas. Generator v1 / Scanner v1.4 / policy v0.1. Accessed 2026-05-28. https://www.mecfsatlas.com/evidence/douche-aourik-2003-detection-enterovirus
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