Salehi, Hassan, Salehi, Marziyeh, Roghanian, Rasoul et al. · Advanced biomedical research · 2016 · DOI
This study compared two different tests for diagnosing infectious mononucleosis (IM), a viral infection caused by Epstein-Barr virus (EBV). Researchers tested 100 patients suspected of having IM and 100 healthy people using two methods: a blood test (ELISA) and a genetic test (PCR). The blood test was easier, faster, and cheaper than the genetic test, making it a better choice for initial diagnosis.
Since EBV infection is considered a potential trigger or contributor to ME/CFS in some patients, accurate and accessible diagnostic methods are important. This study supports using ELISA as a practical screening tool, which could improve early identification of EBV-related illness and help researchers better understand the relationship between acute EBV infection and subsequent chronic fatigue development.
This study does not prove that EBV causes ME/CFS, nor does it establish whether acute EBV infection leads to chronic fatigue syndrome. It also does not demonstrate that ELISA is more accurate than PCR—only that it is more practical and cost-effective. The presence of EBV DNA or antibodies in healthy controls (20-46%) suggests EBV exposure is common and does not necessarily indicate active infectious mononucleosis.
About the PEM badge: “PEM required” means post-exertional malaise was an explicit required diagnostic criterion for participant inclusion in this study — not that PEM was studied, observed, or discussed. Studies using criteria that do not require PEM (e.g. Fukuda, Oxford) are tagged “PEM not required”. How the atlas works →
The first block is for the primary paper and is the citation you should use in research work. The atlas-snapshot line only applies if you are specifically referring to this atlas’s reading of the paper on the date shown.
Primary citation
Salehi, Hassan, Salehi, Marziyeh, Roghanian, Rasoul, Bozari, Majid, Taleifard, Shirin, Salehi, Mohamad Mahdi, et al. (2016). Comparison of serological and molecular test for diagnosis of infectious mononucleosis.. Advanced biomedical research. https://doi.org/10.4103/2277-9175.183144
BibTeX
@article{mecfsatlas-salehi-2016-comparison-serological,
author = {Salehi, Hassan and Salehi, Marziyeh and Roghanian, Rasoul and Bozari, Majid and Taleifard, Shirin and Salehi, Mohamad Mahdi and Salehi, Maryam},
title = {Comparison of serological and molecular test for diagnosis of infectious mononucleosis.},
journal = {Advanced biomedical research},
year = {2016},
doi = {10.4103/2277-9175.183144},
note = {PubMed: 27308267},
url = {https://www.mecfsatlas.com/evidence/salehi-2016-comparison-serological},
}Atlas snapshot reference
ME/CFS Atlas. Generator v1 / Scanner v1.4 / policy v0.1. Accessed 2026-05-28. https://www.mecfsatlas.com/evidence/salehi-2016-comparison-serological
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