ME/CFS Evidence Browser — Classified Studies on Myalgic Encephalomyelitis

2423 studies

141–160 of 2,423 studies

E3 PreliminaryDiagnosticPEM unclearMethods-Paper

Quantitative Proteomics on Immune Cells of ME/CFS Patients Using SWATH-MS.

Kumar, Abhishek, Peppercorn, Katie, Kleffmann, Torsten·Methods in molecular biology (Clifton, N.J.)·2025

This study describes a detailed scientific method for examining proteins in immune cells taken from the blood of ME/CFS patients. Researchers used advanced technology called SWATH-MS (a type of mass spectrometry) to identify and measure thousands of proteins at once. The paper explains the step-by-step process they used so that other researchers can use the same method to find protein abnormalities in ME/CFS.

Immune System

E3 PreliminaryMechanismPEM requiredMechanistic

Exertional Exhaustion (Post-Exertional Malaise, PEM) Evaluated by the Effects of Exercise on Cerebrospinal Fluid Metabolomics-Lipidomics and Serine Pathway in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome.

Baraniuk, James N·International journal of molecular sciences·2025

This study examined fluid from around the brain and spinal cord in ME/CFS patients and healthy controls, measuring chemical substances before and after exercise. Researchers found that ME/CFS patients have abnormal levels of certain chemicals related to energy production and brain function, especially after exercise. The study suggests that exercise may trigger harmful changes in brain chemistry in ME/CFS patients that don't happen in healthy people, which could explain post-exertional malaise (the worsening of symptoms after activity).

Post-Exertional Malaise

E3 PreliminaryReviewPEM not requiredReview-Narrative

A new anchor point for gut microbiome to regulate complications of allogeneic hematopoietic stem cell transplantation: oxidative stress.

Bai, Wenxuan, Jiang, Tiebin, Tang, Lanlan et al.·Experimental hematology·2025

This review examines how bone marrow stem cell transplants can cause complications through a process called oxidative stress (cellular damage from harmful molecules). The authors explain how gut bacteria and their chemical byproducts may help protect the body by reducing this oxidative stress, potentially preventing or lessening serious complications like infections, fatigue, and organ damage that can occur after transplantation.

Immune System

E3 PreliminaryReviewPEM unclearReview-Narrative

Mitochondrial Dysfunction in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome.

Syed, Abu Mohammad, Karius, Alexander K, Ma, Jin et al.·Physiology (Bethesda, Md.)·2025

This review examines how mitochondria—the parts of cells that produce energy—may not be working properly in ME/CFS patients. The authors suggest that problems with energy production in cells could explain why people with ME/CFS feel severely exhausted after physical activity. They also note that similar energy problems appear in long COVID, which suggests both conditions may share a common underlying cause.

Severe MELong COVID OverlapPost-Exertional Malaise

E3 PreliminaryReviewPEM not requiredReview-Narrative

Cerebral Blood Flow in Orthostatic Intolerance.

Khan, Muhammad Shahzeb, Miller, Amanda J, Ejaz, Arooba et al.·Journal of the American Heart Association·2025

When you stand up, your body normally adjusts blood flow to keep your brain supplied with oxygen. Some people have problems with this adjustment, causing dizziness, fatigue, or fainting—a condition called orthostatic intolerance. This review explains that many ME/CFS and long COVID patients have reduced blood flow to the brain when standing, even though their heart rate and blood pressure look normal on standard tests. The authors review different tools doctors can use to actually measure brain blood flow, rather than just relying on heart rate and blood pressure readings.

Autonomic Nervous System

E3 PreliminaryBehavioralPEM unclearObservational

The Psychological Benefits of Forest Bathing in Individuals with Fibromyalgia and Chronic Fatigue Syndrome/Myalgic Encephalomyelitis: A Pilot Study.

Serrat, Mayte, Royuela-Colomer, Estíbaliz, Alonso-Marsol, Sandra et al.·Healthcare (Basel, Switzerland)·2025

This study looked at whether spending time walking quietly in a forest could help people with ME/CFS and fibromyalgia feel better. Forty-four participants took a 3-hour guided walk through a Mediterranean forest, and researchers measured their pain, fatigue, anxiety, and mood before and after. Most symptoms improved after the walk, especially anxiety and negative feelings, though pain levels didn't change significantly.

E3 PreliminaryMechanismPEM requiredPreprintPreprint

Heightened innate immunity may trigger chronic inflammation, fatigue and post-exertional malaise in ME/CFS.

Che, Xiaoyu, Ranjan, Amit, Guo, Cheng et al.·medRxiv : the preprint server for health sciences·2025

This study found that people with ME/CFS may have an overactive immune system that responds too strongly to germs and infections. The research showed problems with how the body produces energy and manages inflammation, and these problems got worse after exercise in ME/CFS patients but not in healthy people. The findings suggest multiple biological systems are working abnormally in ME/CFS, which could help doctors develop better treatments.

Energy MetabolismImmune SystemPost-Exertional Malaise

E3 PreliminaryReviewPEM unclearReview-Narrative

Review of Neuroimaging Methods in ME/CFS.

Thapaliya, Kiran, Inderyas, Maira, Barnden, Leighton·Methods in molecular biology (Clifton, N.J.)·2025

This review examines different brain imaging techniques that have been used to study ME/CFS and detect changes in the brain. Researchers have used three main types of imaging: MRI (which takes detailed pictures of brain structure), PET and SPECT (which show how brain activity and chemicals function). These studies have found subtle but measurable differences in the brains of people with ME/CFS compared to healthy controls.

E3 PreliminaryCohortPEM unclearCase-Control

HERV Dysregulation in a Case of Myalgic Encephalomyelitis and Multiple Sclerosis Responsive to Rituximab.

Martín-Martínez, Eva, Gil-Perotin, Sara, Giménez-Orenga, Karen et al.·International journal of molecular sciences·2025

This study describes one patient who had ME/CFS and was later diagnosed with multiple sclerosis (MS). Doctors discovered that his blood had unusual patterns of human endogenous retroviruses (HERVs)—ancient viral-like sequences in our DNA that can sometimes affect immune function. Importantly, this patient improved significantly after receiving rituximab, a treatment that modifies the immune system, even though standard treatments had not worked for him.

Immune System

E3 PreliminaryReviewPEM not requiredReview-Narrative

Myopraktikk (NO): A Narrative Review and Conceptual Hypothesis on Intrafasciomembranal Fluid Pressure, Biotensegrity, and Immediate Remote Myofascial Responses.

Hopen, Stig Runar, Mjøen, Tor Arne·Cureus·2025

This article proposes a new theory about muscle pain and stiffness called Myopraktikk, which focuses on fluid pressure building up in the spaces around muscles. The authors suggest that when fluid pressure increases in these areas, it can cause muscles to feel tight, knotted, and painful—similar to what happens in conditions like ME/CFS and fibromyalgia. According to their hypothesis, releasing this pressure through manual treatment may help reduce symptoms in both the treated area and elsewhere in the body.

Pain and Sensitization

E3 PreliminaryMechanismPEM requiredMechanistic

Extracellular vesicle proteomics uncovers energy metabolism, complement system, and endoplasmic reticulum stress response dysregulation postexercise in males with myalgic encephalomyelitis/chronic fatigue syndrome.

Glass, Katherine A, Giloteaux, Ludovic, Zhang, Sheng et al.·Clinical and translational medicine·2025

Researchers studied tiny particles called extracellular vesicles in the blood of ME/CFS patients before and after exercise. They found that in people with ME/CFS, these particles show signs of energy problems, immune overactivity, and cellular stress after exertion—changes that were linked to post-exertional malaise (the worsening of symptoms after activity). These findings suggest new targets for treatment that could help improve recovery and energy in ME/CFS.

BiomarkersPost-Exertional Malaise

E3 PreliminaryMechanismPEM unclearMechanistic

A network medicine approach to investigating ME/CFS pathogenesis in severely ill patients: a pilot study.

Hung, Li-Yuan, Wu, Chan-Shuo, Chang, Chia-Jung et al.·Frontiers in human neuroscience·2025

Researchers used advanced computer analysis to examine genetic information from people with severe ME/CFS, looking for patterns in how genes work together. They found that ME/CFS involves multiple body systems working incorrectly, particularly the immune and nervous systems, and discovered connections to viral infections like EBV and COVID-19. The study also found clues about why more women than men develop ME/CFS, relating to how estrogen affects the body.

Immune System

E3 PreliminaryDiagnosticPEM unclearMethods-Paper

Deep Immunophenotyping in ME/CFS Using Spectral Flow Cytometry.

Gibson, Anton, Chometon, Thaize Q, Damani, Tanvi et al.·Methods in molecular biology (Clifton, N.J.)·2025

Researchers developed a new laboratory technique to examine immune cells in the blood of ME/CFS patients in much greater detail than previously possible. Using advanced flow cytometry, they simultaneously measured over 40 different markers on individual immune cells, allowing them to create a detailed map of immune system abnormalities in ME/CFS. This technique is like upgrading from a simple photograph to a high-resolution image, revealing much more about how immune cells are behaving.

Immune System

E3 PreliminaryReviewPEM unclearReview-Narrative

Autoantibody targeting therapies in post COVID syndrome and myalgic encephalomyelitis/chronic fatigue syndrome.

Wohlrab, Felix, Eltity, Mailam, Ufer, Friederike et al.·Expert opinion on biological therapy·2025

This article reviews how certain immune system proteins called autoantibodies—which the body mistakenly makes against itself—may contribute to both long COVID and ME/CFS. The authors discuss different treatment approaches that aim to reduce or neutralize these harmful autoantibodies. This editorial examines the current evidence and potential of these new therapies to help patients with these conditions.

Immune System

E3 PreliminaryCommentaryPEM unclearEditorial

Reframing Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): Biological Basis of Disease and Recommendations for Supporting Patients.

Agarwal, Priya, Friedman, Kenneth J·Healthcare (Basel, Switzerland)·2025

This article is a special essay about ME/CFS that was written by a medical student and selected as the best submission in a scholarship competition. It explains what we currently know about how ME/CFS affects the body and offers practical advice for doctors on how to better care for ME/CFS patients. The essay highlights that ME/CFS is a serious, worldwide health problem affecting millions of people, many of whom haven't been properly diagnosed.

E3 PreliminaryDiagnosticPEM unclearMethods-Paper

Application of DNA Methylome Analysis to Patients with ME/CFS.

Peppercorn, Katie, Edgar, Christina D, Al Momani, Suzan et al.·Methods in molecular biology (Clifton, N.J.)·2025

This study describes a technique called RRBS that examines how chemical tags on our DNA (called methylation) are different in ME/CFS patients compared to healthy people. These tags act like switches that turn genes on or off, and abnormal patterns might help explain why ME/CFS causes such severe symptoms. The researchers show how this method can be used to identify patterns that could eventually lead to better diagnostic tests or ways to track how the illness changes over time.

Biomarkers

E3 PreliminaryCohortPEM unclearCase-Control

Clinically Meaningful Improvements in Long COVID Symptoms Following Ketogenic Metabolic Therapy Combined with Lifestyle Interventions-A Clinical Case Report and Review of the Literature.

Colgan, Dana Dharmakaya, Stadler, Diane D, Hope, Aluko A et al.·Case reports in clinical medicine·2025

This study describes one patient with Long COVID who experienced significant improvements in their symptoms after following a special diet (ketogenic diet) combined with lifestyle changes including exercise, better sleep schedules, and stress-reduction practices delivered remotely. The authors reviewed existing research suggesting this combined approach might help address underlying problems in Long COVID and support the body's ability to handle stress.

E3 PreliminaryBehavioralPEM not requiredCross-Sectional

Mixed methods study of views and experience of non-hospitalised individuals with long COVID of using pacing interventions.

McMullan, Christel, Haroon, Shamil, Turner, Grace et al.·Scientific reports·2025

This study asked people with Long COVID about their experiences using 'pacing'—a strategy to manage energy by balancing activity and rest. Researchers gave questionnaires to 28 people and interviewed 19 of them. Most participants found pacing helpful for planning activities and staying motivated, though some found it complicated and difficult to follow without clearer guidance.

E3 PreliminaryGuidelinePEM requiredGuideline

Multidisciplinary collaborative guidance on the assessment and treatment of patients with Long COVID: A compendium statement.

Cheng, Abby L, Herman, Eric, Abramoff, Benjamin et al.·PM & R : the journal of injury, function, and rehabilitation·2025

This is a comprehensive clinical guide created by over 40 Long COVID treatment centers to help doctors evaluate and care for Long COVID patients. The guide covers how to assess symptoms, manage common problems like fatigue and brain fog, and help patients return to work or daily activities. It emphasizes that Long COVID is real, takes it seriously, and recommends treatments based on what doctors currently know while acknowledging many unknowns remain.

Post-Exertional Malaise

E3 PreliminaryCommentaryPEM unclearEditorial

Chronic Fatigue in Cancer Survivorship: Psychiatry Versus Oncology or Psychiatry with Oncology?

Kafetzopoulos, Vasilios, Pittaka, Maria, Ioannidis, Georgios et al.·Current oncology reports·2025

This review examines fatigue in breast cancer survivors and how it's treated. The authors found that non-drug treatments like exercise, talk therapy, and mindfulness work best for cancer-related fatigue. They point out that cancer doctors and psychiatrists approach fatigue differently, and suggest that a combined team approach—mixing cancer care with mental health support—would help patients most.

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Quantitative Proteomics on Immune Cells of ME/CFS Patients Using SWATH-MS.

Exertional Exhaustion (Post-Exertional Malaise, PEM) Evaluated by the Effects of Exercise on Cerebrospinal Fluid Metabolomics-Lipidomics and Serine Pathway in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome.

A new anchor point for gut microbiome to regulate complications of allogeneic hematopoietic stem cell transplantation: oxidative stress.

Mitochondrial Dysfunction in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome.

Cerebral Blood Flow in Orthostatic Intolerance.

The Psychological Benefits of Forest Bathing in Individuals with Fibromyalgia and Chronic Fatigue Syndrome/Myalgic Encephalomyelitis: A Pilot Study.

Heightened innate immunity may trigger chronic inflammation, fatigue and post-exertional malaise in ME/CFS.

Review of Neuroimaging Methods in ME/CFS.

HERV Dysregulation in a Case of Myalgic Encephalomyelitis and Multiple Sclerosis Responsive to Rituximab.

Myopraktikk (NO): A Narrative Review and Conceptual Hypothesis on Intrafasciomembranal Fluid Pressure, Biotensegrity, and Immediate Remote Myofascial Responses.

Extracellular vesicle proteomics uncovers energy metabolism, complement system, and endoplasmic reticulum stress response dysregulation postexercise in males with myalgic encephalomyelitis/chronic fatigue syndrome.

A network medicine approach to investigating ME/CFS pathogenesis in severely ill patients: a pilot study.

Deep Immunophenotyping in ME/CFS Using Spectral Flow Cytometry.

Autoantibody targeting therapies in post COVID syndrome and myalgic encephalomyelitis/chronic fatigue syndrome.

Reframing Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): Biological Basis of Disease and Recommendations for Supporting Patients.

Application of DNA Methylome Analysis to Patients with ME/CFS.

Clinically Meaningful Improvements in Long COVID Symptoms Following Ketogenic Metabolic Therapy Combined with Lifestyle Interventions-A Clinical Case Report and Review of the Literature.

Mixed methods study of views and experience of non-hospitalised individuals with long COVID of using pacing interventions.

Multidisciplinary collaborative guidance on the assessment and treatment of patients with Long COVID: A compendium statement.

Chronic Fatigue in Cancer Survivorship: Psychiatry Versus Oncology or Psychiatry with Oncology?

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Neuroinflammation Evidence

Metabolism & Mitochondria

Biomarkers

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