Moriya, Junji, He, Qiang, Uenishi, Hiroaki et al. · BioMed research international · 2015 · DOI
Researchers injected mice with bacterial antigens from Brucella abortus to create an animal model of ME/CFS. They found that at high doses, the injections caused temporary anemia (low red blood cells), but at moderate doses, anemia did not develop. Importantly, in both groups, the mice showed persistent low activity levels for weeks after treatment stopped, suggesting the injections could trigger lasting fatigue-like symptoms without necessarily causing anemia.
This research demonstrates that bacterial antigen exposure can produce persistent fatigue-like symptoms in animal models independent of anemia, supporting the hypothesis that post-infectious triggers may contribute to ME/CFS pathogenesis. The moderate-dose protocol without anemia induction may better reflect human disease and could serve as a tool for testing treatments targeting fatigue mechanisms rather than hematologic dysfunction.
This study does not prove that Brucella infection causes human ME/CFS, nor does it establish the mechanisms by which antigen exposure produces fatigue symptoms. The observation of low activity does not confirm this is truly analogous to human ME/CFS fatigue, post-exertional malaise, or associated symptoms like cognitive dysfunction. It also cannot determine whether anemia is necessary or irrelevant to CFS pathogenesis in humans.
About the PEM badge: “PEM required” means post-exertional malaise was an explicit required diagnostic criterion for participant inclusion in this study — not that PEM was studied, observed, or discussed. Studies using criteria that do not require PEM (e.g. Fukuda, Oxford) are tagged “PEM not required”. How the atlas works →
The first block is for the primary paper and is the citation you should use in research work. The atlas-snapshot line only applies if you are specifically referring to this atlas’s reading of the paper on the date shown.
Primary citation
Moriya, Junji, He, Qiang, Uenishi, Hiroaki, Akazawa, Sumiyo, Yamakawa, Jun-Ichi, Kobayashi, Junji, et al. (2015). Induction Murine Models of Chronic Fatigue Syndrome by Brucella abortus Antigen Injections: Is Anemia Induced or Not?. BioMed research international. https://doi.org/10.1155/2015/191489
BibTeX
@article{mecfsatlas-moriya-2015-induction-murine,
author = {Moriya, Junji and He, Qiang and Uenishi, Hiroaki and Akazawa, Sumiyo and Yamakawa, Jun-Ichi and Kobayashi, Junji and Ishigaki, Yasuhito},
title = {Induction Murine Models of Chronic Fatigue Syndrome by Brucella abortus Antigen Injections: Is Anemia Induced or Not?},
journal = {BioMed research international},
year = {2015},
doi = {10.1155/2015/191489},
note = {PubMed: 26171388},
url = {https://www.mecfsatlas.com/evidence/moriya-2015-induction-murine},
}Atlas snapshot reference
ME/CFS Atlas. Generator v1 / Scanner v1.4 / policy v0.1. Accessed 2026-05-25. https://www.mecfsatlas.com/evidence/moriya-2015-induction-murine
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