Tang, Ning, Frank, Andrea, Leckie, Gregor et al. · Journal of virological methods · 2012 · DOI
Researchers developed laboratory tests to detect XMRV, a virus that was proposed as a possible cause of ME/CFS. They tested these new detection methods on blood, urine, and other samples from healthy people, HIV-positive people, and prostate cancer patients. The tests worked well in the lab, but when applied to real patient samples, they did not find any confirmed cases of XMRV.
This study is important because it represents a rigorous attempt to develop and validate sensitive tools for detecting XMRV in ME/CFS patients and other populations. The negative findings in large clinical sample sets provide crucial evidence regarding the actual prevalence of XMRV in human disease, helping to inform the scientific debate about XMRV's role in ME/CFS etiology.
This study does not prove that XMRV is definitively absent from ME/CFS patients, as the screened cohort was not specifically recruited for ME/CFS diagnosis. The negative results reflect the prevalence of XMRV in the particular populations tested (blood donors, HIV-positive individuals, prostate cancer patients), not necessarily the entire ME/CFS population. Cross-reactivity with mouse DNA suggests the assays have some limitations in specificity.
About the PEM badge: “PEM required” means post-exertional malaise was an explicit required diagnostic criterion for participant inclusion in this study — not that PEM was studied, observed, or discussed. Studies using criteria that do not require PEM (e.g. Fukuda, Oxford) are tagged “PEM not required”. How the atlas works →
The first block is for the primary paper and is the citation you should use in research work. The atlas-snapshot line only applies if you are specifically referring to this atlas’s reading of the paper on the date shown.
Primary citation
Tang, Ning, Frank, Andrea, Leckie, Gregor, Hackett, John, Simmons, Graham, Busch, Michael, et al. (2012). Development of sensitive single-round pol or env RT-PCR assays to screen for XMRV in multiple sample types.. Journal of virological methods. https://doi.org/10.1016/j.jviromet.2011.10.010
BibTeX
@article{mecfsatlas-tang-2012-development-sensitive,
author = {Tang, Ning and Frank, Andrea and Leckie, Gregor and Hackett, John and Simmons, Graham and Busch, Michael and Abravaya, Klara},
title = {Development of sensitive single-round pol or env RT-PCR assays to screen for XMRV in multiple sample types.},
journal = {Journal of virological methods},
year = {2012},
doi = {10.1016/j.jviromet.2011.10.010},
note = {PubMed: 22057262},
url = {https://www.mecfsatlas.com/evidence/tang-2012-development-sensitive},
}Atlas snapshot reference
ME/CFS Atlas. Generator v1 / Scanner v1.4 / policy v0.1. Accessed 2026-05-27. https://www.mecfsatlas.com/evidence/tang-2012-development-sensitive
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